Technique

Red Blood Cell-Ready Gene is inno-train's product line for analysis of erythrocyte blood groups based on the SSP-PCR method. Therefore these diagnostic systems represent the ideal complement to serolgoical blood group typing. The modular kit design offers an extensive choice for a gradual typing system depending on your problem.

The evaluation is performed by standard agarose gel electrophoresis. As an internal PCR control each tube contains primers for amplification of the Human Growth Hormone (HGH). If no specific product is present after PCR, the amplificate of this positive control must be clearly detectable.

Advantages of the RBC-Ready Gene System

• high flexibility due to modular product concept
• easy and fast test performance
• safety with polytransfused patients
• clarification of questionable RHD/RHCE
• negative controls included
• coloured primer mixes for better visibility
• CE certified

AB0 Genotyping

Patients with weak AB0 expression or with a questionable pattern of their isoagglutinins can be retyped with RBC-Ready Gene AB0 / AB0 Subtype up to very weakly expressed antigens.

The flexible format of RBC-Ready Gene allows the performance of both kits in parallel, or only the subtype depending on your requirements.

Genotyping of Rare Blood Groups

In many cases antisera against rare blood groups are not available or difficult to identify and that can be directed against rare blood groups. RBC-Ready Gene Rare ID detects rare blood group alleles without a doubt and in an economical way.

In times of a globalized world the demand for blood units with rare blood group alleles is increasing. Our screening concepts for rare blood group alleles support you in fining the suitable blood unit. You have the choice: the RBC-Ready Gene Rare Screen system screens with only one reaction for five different blood group alleles. The RBC-Ready Gene 4-Screen seaches in four reaction of seven different rare blood group alleles in parallel with the confirmation of D positivity or D negativity.

Further identification of the detected rare allele after such a screening test can be completed with the RBC-Ready Gene Rare ID system.

Genotyping of the KELL, KIDD, DUFFY and MNS systems

Different hemolytic diseases e. g. sickle cell anemia or thalassemia, but also complex courses of diseases following multiple operations due to accidents or chronic diseases require continuous blood transfusions. As a result the recipients have a mixture of blood that makes blood group detection difficult, expecially of the blood groups Kell, Kidd, Duffy and MNS. If further transfusions are necessary molecular blood group detection gives clear result because the colume of donor DNA in the transfusedblood units dies not influence the test.

RBC-Ready Gene also gives you safety in typing patients who have generated allo- or autoanitbodies and/or show a positive DAT in serological typing.

Beside the standard RBC-Ready Gene MNS and RBC-Ready Gene KKD test systems we offer the RBC-Ready Gene KELplus kit for detection of further Kell alleles, as well as the RBC-Ready Gene JKplusFY for detection of further Kidd alleles in combination with Duffy detection.

RHD Genotyping

For clarification of serologically weak D typings in patients and donors inno-train's system RBC-Ready Gene CDE and RBC-Ready Gene D weak can be used individually or in combination. Ambiguous samples are tested specifically for D categroies, D partial and D weak and can therefor be clearly characterized.

Molecular retyping of samples are serologically D negative with a C or E antigen show a D positivity (DEL, D weak, D variant) in rare cases.