• To reduce ambiguities two amplifications are carried out in parallel in order to separate many heterozygous allele combinations in the first step
• Gel Electrophoresis
• Purification of the PCR products using ExoSAP-ITTM
• 6 sequence reactions per HLA Class I Locus sequence
• 3-5 sequence reactions per HLA Class II Locus sequence
• The combination of forward and reverse reactions takes place according to positive PCR amplification
• Precipitation with IT-Prec-Buffer
• Loading of the capillary sequencer
• Analysis with HLA-HiType software

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